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KMID : 1234920220220010030
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Journal of Korean Oriental Association for Study of Obesity
2022 Volume.22 No. 1 p.30 ~ p.37
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Extract of Ranunculus sceleratus Reduced Adipogenesis by Inhibiting AMPK Pathway in 3T3-L1 Preadipocytes
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Kim Yae-Ji
Cho Sung-Pil
Lee Hui-Ju
Hong Geum-Lan
Kim Kyung-Hyun
Ryu Si-Yun
Jung Ju-Young
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Abstract
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Objectives: Adipogenesis is the process by which pre-adipocytes are differentiated into adipocytes. It also plays an important role in adipocyte formation and lipid accumulation.
Ranunculus sceleratus (R. sceleratus) extracts are used for the treatment of various diseases such as hepatitis, jaundice, and tuberous lymphadenitis in oriental medicine.
However, its effect on adipogenesis has not yet been studied. In this study, we investigated the effects of R. sceleratus on adipogenesis in 3T3-L1 cells.
Methods: Cells were treated with 50, 100, and 200 μg/ml of R. sceleratus and cell viability was evaluated. To differentiate the 3T3-L1 preadipocytes, a 3-isobutyl-1-methylxanthine, dexamethasone, and insulin (MDI) solution were used. The accumulation of lipid droplets was determined by Oil Red O staining. The expression levels of adipogenesis-related proteins were also determined.
Results: MDI solution differentiated the preadipocytes into adipocytes and accumulation of lipids was observed in the differentiated 3T3-L1 cells. Interestingly, the amount of lipid droplets was reduced after R. sceleratus treatment. In addition, the expression levels of key adipogenic transcription factors, such as CCAAT/enhancer-binding proteins-α (C/EBP- α) and peroxisome proliferator-activated receptors-γ (PPAR-γ) were also reduced after R. sceleratus treatment. Furthermore, R. sceleratus increased AMP-activated kinase (AMPK) phosphorylation and decreased sterol regulatory element-binding protein-1 expression.
Conclusions: Our results showed that R. sceleratus reduced preadipocyte differentiation by inhibiting C/EBP-α and PPAR-γ levels via the AMPK pathway. Therefore, we suggest that R. sceleratus may be potentially used as an anti-adipogenic agent.
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KEYWORD
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3T3-L1 cells, Adipogenesis, CCAAT/enhancer-binding proteins-α, PPAR gramma, AMP-activated protein kinases, Ranunculus sceleratus
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